Search results for "Segmental patterning"

showing 3 items of 3 documents

The Drosophila Hox gene Ultrabithorax acts both in muscles and motoneurons to orchestrate formation of specific neuromuscular connections

2016

Hox genes are known to specify motoneuron pools in the developing vertebrate spinal cord and to control motoneuronal targeting in several species. However, the mechanisms controlling axial diversification of muscle innervation patterns are still largely unknown. We present data showing that the Drosophila Hox gene Ultrabithorax (Ubx) acts in the late embryo to establish target specificity of ventrally projecting RP motoneurons. In abdominal segments A2 to A7, RP motoneurons innervate the ventrolateral muscles VL1-4, with VL1 and VL2 being innervated in a Wnt4-dependent manner. In Ubx mutants, these motoneurons fail to make correct contacts with muscle VL1, a phenotype partially resembling t…

0301 basic medicineCell typeEmbryo Nonmammaliananimal structuresNeuromuscular JunctionGenes InsectMuscle DevelopmentNeuromuscular junctionAnimals Genetically ModifiedHox genes03 medical and health sciencesWNT4MorphogenesismedicineAnimalsDrosophila ProteinsHox geneWnt Signaling PathwayMolecular BiologyTranscription factorUltrabithoraxHomeodomain ProteinsMotor NeuronsGeneticsbiologyMusclesmusculoskeletal neural and ocular physiologyfungiGenes HomeoboxGene Expression Regulation Developmentalbiology.organism_classificationMuscle innervationSegmental patterningCell biologyMotoneuronsDrosophila melanogaster030104 developmental biologymedicine.anatomical_structurenervous system209embryonic structuresDrosophilaWnt signalling pathwayDrosophila melanogasterDrosophila ProteinTranscription FactorsResearch ArticleDevelopmental BiologyDevelopment
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Gene expression profiles uncover individual identities of gnathal neuroblasts and serial homologies in the embryonic CNS of Drosophila.

2015

The numbers and types of progeny cells generated by neural stem cells in the developing CNS are adapted to its region-specific functional requirements. In Drosophila, segmental units of the CNS develop from well-defined patterns of neuroblasts. Here we constructed comprehensive neuroblast maps for the three gnathal head segments. Based on the spatiotemporal pattern of neuroblast formation and the expression profiles of 46 marker genes (41 transcription factors), each neuroblast can be uniquely identified. Compared with the thoracic ground state, neuroblast numbers are progressively reduced in labial, maxillary and mandibular segments due to smaller sizes of neuroectodermal anlagen and, part…

0301 basic medicineCentral Nervous SystemGenetic Markersanimal structuresSerial homologyCell CountGenes InsectBiology03 medical and health sciences0302 clinical medicineNeuroblastNeural Stem CellsNeuroblastsAbdomenAnimalsCell LineageHox geneMolecular Biologyreproductive and urinary physiologyfungiAnatomyThoraxGene expression profileNeuromereStem Cells and RegenerationEmbryonic stem cellNeural stem cellCell biology103Segmental patterning030104 developmental biologyDrosophila melanogasternervous systemVentral nerve cordDrosophila brainembryonic structuresDeformedTranscriptomeGanglion mother cell030217 neurology & neurosurgeryDevelopmental BiologyDevelopment (Cambridge, England)
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Abdominal-B and caudal inhibit the formation of specific neuroblasts in the Drosophila tail region

2013

The central nervous system of Drosophila melanogaster consists of fused segmental units (neuromeres), each generated by a characteristic number of neural stem cells (neuroblasts). In the embryo, thoracic and anterior abdominal neuromeres are almost equally sized and formed by repetitive sets of neuroblasts, whereas the terminal abdominal neuromeres are generated by significantly smaller populations of progenitor cells. Here we investigated the role of the Hox gene Abdominal-B in shaping the terminal neuromeres. We show that the regulatory isoform of Abdominal-B (Abd-B.r) not only confers abdominal fate to specific neuroblasts (e.g. NB6-4) and regulates programmed cell death of several proge…

Central Nervous SystemTailanimal structuresCNS developmentCellular differentiationParaHoxApoptosisBiologyTerminal neuromeresAbdominal-BHox genesNeural Stem CellsNeuroblastNeuroblastsImage Processing Computer-AssistedAnimalsDrosophila ProteinsHox geneMolecular BiologyIn Situ HybridizationDNA PrimersHomeodomain ProteinsfungiCell DifferentiationStem Cells and RegenerationNeuromereImmunohistochemistryMolecular biologyNeural stem cellSegmental patterningDrosophila melanogasterMicroscopy Fluorescencenervous systemembryonic structuresCaudalDrosophilaGanglion mother cellDrosophila ProteinTranscription FactorsDevelopmental BiologyDevelopment
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